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Thermal equilibration was then conducted for 20 ps, gradually increasing the temperature from 100 K to 298 K using the Langevin thermostat (collision frequency of 1 ps 1 )

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A 2004 follow-up confirmed the fibroblast data and added observations on cell division capacity

At the ultrastructural level, irregular and immature elastin fibers and fibers devoid of microfibrils were observed. These findings suggested that both elastic fiber abnormalities and reduced collagen content contribute to the clinical features seen in patients with clEDS. There were no consistent electron microscopy (EM) findings in the ten patients in our cohort who had EM performed